Github.com/aryeelab/nanopore-tools/dorado_basecall

system · March 3, 2023, 2:19am

Oxford nanopore tools

Installing dependencies

Install Homebrew

Install cromwell

brew install cromwell

Install Docker

Config for 5mC and 6mA

github.com/nanoporetech/rerio

Methylation (5mC & 6mA) call for MinION R10.3 reads

opened 05:47AM - 03 Feb 21 UTC

yfuruta

Hi, I am wondering is there any model available for methylation (5mC & 6mA) c…all for MinION R10.3 reads. Does a config file included in guppy or any model in rerio work? If not, any information about the schedule for publicizing such model? I just in case tried both dna_r10.3_450bps_fast.cfg and dna_r10.3_450bps_hac.cfg included guppy, but called fast5 didn't include ModBaseProbs table. For MinION R9.4.1 reads, Megalodon and res_dna_r941_min_modbases-all-context_v001.cfg in rerio worked perfectly!! I'm looking forward to doing same analysis with MinION R10.3 reads. Thank you so much in advance! Best, Yoshi

Methylation (5mC & 6mA) call for MinION R10.3 reads #20
For MinION R9.4.1 reads, Megalodon and res_dna_r941_min_modbases-all-context_v001.cfg in rerio worked perfectly!! I’m looking forward to doing

This is a companion discussion topic for the original entry at github.com/aryeelab/nanopore-tools/dorado_basecall