LRAA - Long Read Alignment Assembler
** Under Development **
Utility for resolving and/or quantification of isoforms from aligned PacBio Iso-seq reads.
Isoform Discovery
Given a bam file from aligned reads (using minimap2), perform isoform discovery like so:
LRAA --genome genome.fasta --bam aligned_IsoSeq.mm2.bam
Isoform Quantification
LRAA --genome genome.fasta --bam aligned_IsoSeq.mm2.bam --gtf target_isoforms.gtf --quant_only
This is a companion discussion topic for the original entry at github.com/MethodsDev/LongReadAlignmentAssembler/LRAA