github.com/ShalekLab/tcrgo/TCRGO

Description

TCR Recovery for Seq-Well single-cell RNA-seq data. More to be added here.

Requirements

Hardware: macOS (tested), linux (untested) or Windows (untested). Recommended 16GB RAM and at least 50 GB free space on hard drive.
Software: Python >= 3.8.5, pysam == 0.16.0.1, pandas == 1.1.2, bowtie2 >= 2.4.1, samtools >= 1.3.1, Java 12.0.1 (recommended), Drop-Seq Tools >= 2.3.0

Install instructions

It’s best you create a new virtual environment. Example using conda on macOS:


This is a companion discussion topic for the original entry at github.com/ShalekLab/tcrgo/TCRGO